Article
Improving the genome editing efficiency of CRISPR/Cas9 in Arabidopsis and Medicago truncatula.
Planta - 8 Jul 2020
Wolabu Tezera W, Park Jong-Jin, Chen Miao, Cong Lili, Ge Yaxin, Jiang Qingzhen, Debnath Smriti, Li Guangming, Wen Jiangqi, Wang Zengyu
Abstract excerpt
MAIN CONCLUSION: An improved CRISPR/Cas9 system with the Arabidopsis UBQ10 promoter-driven Cas9 exhibits consistently high mutation efficiency in Arabidopsis and M. truncatula. CRISPR/Cas9 is a powerful genome editing technology that has been applied in several crop species for trait improvement due to its simplicity, versatility, and specificity. However, the mutation efficiency of CRISPR/Cas9 in Arabidopsis and...
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