Article
Improving the Genome Editing Efficiency of CRISPR/Cas9 in Melon and Watermelon.
Cells - 28 Oct 2024
Wang Zhuanrong, Wan Lili, Ren Jian, Zhang Na, Zeng Hongxia, Wei Jiaqi, Tang Mi
Abstract excerpt
CRISPR/Cas9 is a powerful genome editing tool for trait improvement in various crops; however, enhancing mutation efficiency using CRISPR/Cas9 in watermelon and melon remains challenging. We designed four CRISPR systems with different sgRNA expression cassettes to target the phytoene desaturase (PDS) gene in melon. The constructed vectors were delivered to host plants using Agrobacterium-mediated transformation....
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