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Article

CRISPR-offinder: a CRISPR guide RNA design and off-target searching tool for user-defined protospacer adjacent motif.

International journal of biological sciences - 1 Jan 2017

Zhao Changzhi, Zheng Xiaoguo, Qu Wubin, Li Guanglei, Li Xinyun, Miao Yi-Liang, Han Xiaosong, Liu Xiangdong, Li Zhenhua, Ma Yunlong, Shao Qianzhi, Li Haiwei, Sun Fei, Xie Shengsong, Zhao Shuhong

Abstract excerpt

Designing efficient and specific CRISPR single-guide RNAs (sgRNAs) is vital for the successful application of CRISPR technology. Currently, a growing number of new RNA-guided endonucleases with a different protospacer adjacent motif (PAM) have been discovered, suggesting the necessity to develop a versatile tool for designing sgRNA to meet the requirement of different RNA-guided DNA endonucleases. Here, we report...

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Identifiers

PMID: 29230095
DOI: 10.7150/ijbs.21312
PMCID: PMC5723913

Genes

PAM

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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