Article
Variants with a low allele frequency detected in genomic DNA affect the accuracy of mutation detection in cell-free DNA by next-generation sequencing.
Cancer - 1 Mar 2018
Wang Jacqueline F, Pu Xingxiang, Zhang Xiaoshan, Chen Ken, Xi Yuanxin, Wang Jing, Mao Xizeng, Zhang Jianhua, Heymach John V, Antonoff Mara B, Hofstetter Wayne L, Mehran Reza J, Rice David C, Roth Jack A, Sepesi Boris, Swisher Stephen G, Vaporciyan Ara A, Walsh Garrett L, Meng Qing H, Shaw Kenna R, Eterovic Agda Karina, Fang Bingliang
Abstract excerpt
BACKGROUND: Next-generation sequencing of cell-free DNA (cfDNA) has been shown to be a useful noninvasive test for detecting mutations in solid tumors. METHODS: Targeted gene sequencing was performed with a panel of 263 cancer-related genes for cfDNA and genomic DNA of peripheral blood mononuclear cells (PBMCs) obtained from presurgical specimens of 6 lung cancer patients, and mutation calls in these samples were...
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