Article
Abundant off-target edits from site-directed RNA editing can be reduced by nuclear localization of the editing enzyme.
RNA biology - 2 Jan 2018
Vallecillo-Viejo Isabel C, Liscovitch-Brauer Noa, Montiel-Gonzalez Maria Fernanda, Eisenberg Eli, Rosenthal Joshua J C
Abstract excerpt
Site-directed RNA editing (SDRE) is a general strategy for making targeted base changes in RNA molecules. Although the approach is relatively new, several groups, including our own, have been working on its development. The basic strategy has been to couple the catalytic domain of an adenosine (A) to inosine (I) RNA editing enzyme to a guide RNA that is used for targeting. Although highly efficient on-target...
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