Article
Improved detection of BRAF V600E using allele-specific PCR coupled with external and internal controllers.
Scientific reports - 23 Oct 2017
Yang Zhao, Zhao Na, Chen Dong, Wei Kun, Su Ning, Huang Jun-Fu, Xu Han-Qing, Duan Guang-Jie, Fu Wei-Ling, Huang Qing
Abstract excerpt
Although traditional allele-specific PCR (tAS-PCR) is a common screening method for BRAF V600E mutations, its lower amplification specificity and mutation selectivity have limited its clinical applications. We hypothesize that these limitations are associated with the weaker specificities of allele-specific primers and the thermodynamic driving forces of DNA polymerase. We used three strategies to circumvent...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.