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Article

A Synthetic Oligo Library and Sequencing Approach Reveals an Insulation Mechanism Encoded within Bacterial σ54 Promoters.

Cell reports - 17 Oct 2017

Levy Lior, Anavy Leon, Solomon Oz, Cohen Roni, Brunwasser-Meirom Michal, Ohayon Shilo, Atar Orna, Goldberg Sarah, Yakhini Zohar, Amit Roee

Abstract excerpt

We use an oligonucleotide library of >10,000 variants to identify an insulation mechanism encoded within a subset of σ54 promoters. Insulation manifests itself as reduced protein expression for a downstream gene that is expressed by transcriptional readthrough. It is strongly associated with the presence of short CT-rich motifs (3-5 bp), positioned within 25 bp upstream of the Shine-Dalgarno (SD) motif of the...

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PMID: 29045849
DOI: 10.1016/j.celrep.2017.09.063

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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