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Article

Quantification of miRNAs Co-Immunoprecipitated with Argonaute Proteins Using SYBR Green-Based qRT-PCR.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018

Phan Hong-Duc, Li Junan, Poi Ming, Nakanishi Kotaro

Abstract excerpt

MicroRNAs (miRNAs) are small non-coding RNAs that trigger post-transcriptional gene silencing. These RNAs need to be associated with the Argonaute proteins to be functional. This assembly begins with loading of a miRNA duplex, followed by the ejection of one of the strands (passenger). The remaining strand (guide) together with the Argonaute protein forms a ribonucleoprotein effector complex (the RNA-induced...

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PMID: 29030839
DOI: 10.1007/978-1-4939-7339-2_2
PMCID: PMC11323259

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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