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Article

Protein Conformational Changes Are Detected and Resolved Site Specifically by Second-Harmonic Generation.

Biophysical journal - 18 Aug 2015

Moree Ben, Connell Katelyn, Mortensen Richard B, Liu C Tony, Benkovic Stephen J, Salafsky Joshua

Abstract excerpt

We present here a straightforward, broadly applicable technique for real-time detection and measurement of protein conformational changes in solution. This method is based on tethering proteins labeled with a second-harmonic generation (SHG) active dye to supported lipid bilayers. We demonstrate our method by measuring the conformational changes that occur upon ligand binding with three well-characterized...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 26287632
DOI: 10.1016/j.bpj.2015.07.016
PMCID: PMC4547196

Genes

DHFR
MBP

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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