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Article

Combined CRISPRi/a-Based Chemical Genetic Screens Reveal that Rigosertib Is a Microtubule-Destabilizing Agent.

Molecular cell - 5 Oct 2017

Jost Marco, Chen Yuwen, Gilbert Luke A, Horlbeck Max A, Krenning Lenno, Menchon Grégory, Rai Ankit, Cho Min Y, Stern Jacob J, Prota Andrea E, Kampmann Martin, Akhmanova Anna, Steinmetz Michel O, Tanenbaum Marvin E, Weissman Jonathan S

Abstract excerpt

Chemical libraries paired with phenotypic screens can now readily identify compounds with therapeutic potential. A central limitation to exploiting these compounds, however, has been in identifying their relevant cellular targets. Here, we present a two-tiered CRISPR-mediated chemical-genetic strategy for target identification: combined genome-wide knockdown and overexpression screening as well as focused,...

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Identifiers

PMID: 28985505
DOI: 10.1016/j.molcel.2017.09.012
PMCID: PMC5640507

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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