Article
Efficient generation of mutations mediated by CRISPR/Cas9 in the hairy root transformation system of Brassica carinata.
PloS one - 1 Jan 2017
Kirchner Thomas W, Niehaus Markus, Debener Thomas, Schenk Manfred K, Herde Marco
Abstract excerpt
A protocol for the induction of site-directed deletions and insertions in the genome of Brassica carinata with CRISPR is described. The construct containing the Cas9 nuclease and the guide RNA (gRNA) was delivered by the hairy root transformation technique, and a successful transformation was monitored by GFP fluorescence. PAGE analysis of an amplified region, presumably containing the deletions and insertions,...
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