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Targeted mutagenesis in cotton (Gossypium hirsutum L.) using the CRISPR/Cas9 system.

Scientific reports - 13 Mar 2017

Chen Xiugui, Lu Xuke, Shu Na, Wang Shuai, Wang Junjuan, Wang Delong, Guo Lixue, Ye Wuwei

Abstract excerpt

The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9 system has been widely used for genome editing in various plants because of its simplicity, high efficiency and design flexibility. However, to our knowledge, there is no report on the application of CRISPR/Cas9-mediated targeted mutagenesis in cotton. Here, we report the genome editing and targeted mutagenesis in upland cotton (Gossypium...

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Identifiers

PMID: 28287154
DOI: 10.1038/srep44304
PMCID: PMC5347080

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