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Article

Analysis of BRCA2 loss of heterozygosity in tumor tissue using droplet digital polymerase chain reaction.

Human pathology - 1 Jul 2014

Cochran Rory L, Cravero Karen, Chu David, Erlanger Bracha, Toro Patricia Valda, Beaver Julia A, Zabransky Daniel J, Wong Hong Yuen, Cidado Justin, Croessmann Sarah, Parsons Heather, Kim Minsoo, Wheelan Sarah J, Argani Pedram, Ho Park Ben

Abstract excerpt

Loss-of-heterozygosity (LOH) analysis of archival tumor tissue can aid in determining the clinical significance of BRCA variants. Here we describe an approach for assessing LOH in formalin-fixed, paraffin-embedded (FFPE) tissues using variant-specific probes and droplet digital polymerase chain reaction (ddPCR). We evaluated LOH in 2 related breast cancer patients harboring a rare missense BRCA2 variant of...

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Identifiers

PMID: 24824029
DOI: 10.1016/j.humpath.2014.03.013
PMCID: PMC4065621

Genes

BRCA2

Variants

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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