Article
Methylation of DNA Ligase 1 by G9a/GLP Recruits UHRF1 to Replicating DNA and Regulates DNA Methylation.
Molecular cell - 17 Aug 2017
Ferry Laure, Fournier Alexandra, Tsusaka Takeshi, Adelmant Guillaume, Shimazu Tadahiro, Matano Shohei, Kirsh Olivier, Amouroux Rachel, Dohmae Naoshi, Suzuki Takehiro, Filion Guillaume J, Deng Wen, de Dieuleveult Maud, Fritsch Lauriane, Kudithipudi Srikanth, Jeltsch Albert, Leonhardt Heinrich, Hajkova Petra, Marto Jarrod A, Arita Kyohei, Shinkai Yoichi, Defossez Pierre-Antoine
Abstract excerpt
DNA methylation is an essential epigenetic mark in mammals that has to be re-established after each round of DNA replication. The protein UHRF1 is essential for this process; it has been proposed that the protein targets newly replicated DNA by cooperatively binding hemi-methylated DNA and H3K9me2/3, but this model leaves a number of questions unanswered. Here, we present evidence for a direct recruitment of...
Topics
- Animals
- CCAAT-Enhancer-Binding Proteins
- DNA
- DNA Ligase ATP
- DNA Methylation
- DNA Replication
- Embryonic Stem Cells
- Epigenesis, Genetic
- HEK293 Cells