Article
Impact of the removal of N-terminal non-structured amino acids on activity and stability of xylanases from Orpinomyces sp. PC-2.
International journal of biological macromolecules - 1 Jan 2018
Ventorim Rafaela Zandonade, de Oliveira Mendes Tiago Antônio, Trevizano Larissa Mattos, Dos Santos Camargos Ana Maria, Guimarães Valéria Monteze
Abstract excerpt
Xylanases catalyze the random hydrolysis of xylan backbone from plant biomass and thus, they have application in the production of biofuels, Kraft pulps biobleaching and feed industry. Here, xylanases derived from Orpinomyces sp. PC-2 were engineered guided by molecular dynamics methods to obtain more thermostable enzymes. Based on these models, 27 amino acid residues from the N-terminal were predicted to reduce...
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