Article
Structural and In Vivo Studies on Trehalose-6-Phosphate Synthase from Pathogenic Fungi Provide Insights into Its Catalytic Mechanism, Biological Necessity, and Potential for Novel Antifungal Drug Design.
mBio - 25 Jul 2017
Miao Yi, Tenor Jennifer L, Toffaletti Dena L, Maskarinec Stacey A, Liu Jiuyu, Lee Richard E, Perfect John R, Brennan Richard G
Abstract excerpt
The disaccharide trehalose is critical to the survival of pathogenic fungi in their human host. Trehalose-6-phosphate synthase (Tps1) catalyzes the first step of trehalose biosynthesis in fungi. Here, we report the first structures of eukaryotic Tps1s in complex with substrates or substrate analogues. The overall structures of Tps1 from Candida albicans and Aspergillus fumigatus are essentially identical and...
Topics
- Antifungal Agents
- Aspergillus fumigatus
- Biocatalysis
- Biofilms
- Candida albicans
- Catalytic Domain
- Drug Design
- Glucosyltransferases
- Humans
- Hyphae
- Mutation
- Protein Binding
- Trehalose