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Enhanced simultaneous overlap extension-PCR by gold nanoparticles.

Nanomedicine : nanotechnology, biology, and medicine - 1 Oct 2017

Yang Wenchao, Cao Xueyan, Li Xinhui

Abstract excerpt

Methods to fuse multiple DNA fragments are extremely useful in synthetic biology and protein engineering. Here, we report a gold nanoparticle-mediated simultaneous overlap extension-PCR (AuNP-mediated SOE-PCR) method that enables the fusion of multiple DNA fragments simultaneously with their amplification in a single reaction using typical PCR conditions. Using greater concentrations of rTaq DNA polymerase and...

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PMID: 28673853
DOI: 10.1016/j.nano.2017.06.016

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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