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Conjugation of fluorescent proteins with DNA oligonucleotides.

Bioconjugate chemistry - 19 May 2010

Lapiene Vidmantas, Kukolka Florian, Kiko Kathrin, Arndt Andreas, Niemeyer Christof M

Abstract excerpt

This work describes the synthesis of covalent ssDNA conjugates of six fluorescent proteins, ECFP, EGFP, E(2)GFP, mDsRed, Dronpa, and mCherry, which were cloned with an accessible C-terminal cystein residue to enable site-selective coupling using a heterobispecific cross-linker. The resulting conjugates revealed similar fluorescence emission intensity to the unconjugated proteins, and the functionality of the...

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PMID: 20481502
DOI: 10.1021/bc900471q

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