Article
Evaluation of whole exome sequencing by targeted gene sequencing and Sanger sequencing.
Clinica chimica acta; international journal of clinical chemistry - 1 Aug 2017
Chang Ya-Sian, Huang Hsien-Da, Yeh Kun-Tu, Chang Jan-Gowth
Abstract excerpt
BACKGROUND: Targeted gene sequencing (TGS) and whole exome sequencing (WES) are being used in clinical testing in laboratories. We compared the performances of TGS and WES using the same DNA samples. METHODS: DNA was extracted from 10 endometrial tumor tissue specimens. Sequencing were performed with an Illumina HiSeq 2000. We randomly selected variants to confirm through Sanger sequencing or mutant-enriched PCR...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.