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Article

Systematic Evaluation of Sanger Validation of Next-Generation Sequencing Variants.

Clinical chemistry - 1 Apr 2016

Beck Tyler F, Mullikin James C, Biesecker Leslie G

Abstract excerpt

BACKGROUND: Next-generation sequencing (NGS) data are used for both clinical care and clinical research. DNA sequence variants identified using NGS are often returned to patients/participants as part of clinical or research protocols. The current standard of care is to validate NGS variants using Sanger sequencing, which is costly and time-consuming. METHODS: We performed a large-scale, systematic evaluation of...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 26847218
DOI: 10.1373/clinchem.2015.249623
PMCID: PMC4878677

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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