Detection of genome-edited mutant clones by a simple competition-based PCR method.

doi:10.1371/journal.pone.0179165

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Article

Detection of genome-edited mutant clones by a simple competition-based PCR method.

PloS one - 1 Jan 2017

Harayama Takeshi, Riezman Howard

Abstract excerpt

Genome editing by the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats / CRISPR-associated protein 9) system is a revolutionary strategy to study gene functions. Since the efficiency of gene disruption in cell culture does not reach 100% typically, cloning of mutant cells is...

Topics

Bacterial Proteins
CRISPR-Associated Protein 9
CRISPR-Cas Systems
DNA Primers
Endonucleases
Gene Knockout Techniques
Genome
HeLa Cells
Humans
Mutation
Polymerase Chain Reaction
RNA Editing
Streptococcus pyogenes

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