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Article

Detection of genome-edited mutant clones by a simple competition-based PCR method.

PloS one - 1 Jan 2017

Harayama Takeshi, Riezman Howard

Abstract excerpt

Genome editing by the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats / CRISPR-associated protein 9) system is a revolutionary strategy to study gene functions. Since the efficiency of gene disruption in cell culture does not reach 100% typically, cloning of mutant cells is often performed to obtain fully mutated cells. Therefore, a method to discriminate accurately mutated clones easily...

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PMID: 28586390
DOI: 10.1371/journal.pone.0179165
PMCID: PMC5460891

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Source receipt: pubmed, retrieved 7 Sept 2026.

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