Article
Quantitative Assessment of Fat Levels in Caenorhabditis elegans Using Dark Field Microscopy.
G3 (Bethesda, Md.) - 7 Jun 2017
Fouad Anthony D, Pu Shelley H, Teng Shelly, Mark Julian R, Fu Moyu, Zhang Kevin, Huang Jonathan, Raizen David M, Fang-Yen Christopher
Abstract excerpt
The roundworm Caenorhabditis elegans is widely used as a model for studying conserved pathways for fat storage, aging, and metabolism. The most broadly used methods for imaging fat in C. elegans require fixing and staining the animal. Here, we show that dark field images acquired through an ordinary light microscope can be used to estimate fat levels in worms. We define a metric based on the amount of light...
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