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Article

Tips on the analysis of phosphatidic acid by the fluorometric coupled enzyme assay.

Analytical biochemistry - 1 Jun 2017

Hassaninasab Azam, Han Gil-Soo, Carman George M

Abstract excerpt

The fluorometric coupled enzyme assay to measure phosphatidic acid (PA) involves the solubilization of extracted lipids in Triton X-100, deacylation, and the oxidation of PA-derived glycerol-3-phosphate to produce hydrogen peroxide for conversion of Amplex Red to resorufin. The enzyme assay is sensitive, but plagued by high background fluorescence from the peroxide-containing detergent and incomplete heat...

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Identifiers

PMID: 28359787
DOI: 10.1016/j.ab.2017.03.020
PMCID: PMC5425298

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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