Article
Assaying plant phosphatidic acid phosphatase activity.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013
Nakamura Yuki
Abstract excerpt
Phosphatidic acid phosphatase (PAP; EC 3.1.3.4) catalyzes the dephosphorylation of phosphatidic acid (PA) to produce diacylglycerol (DAG) and inorganic phosphate. In seed plants, PA plays pivotal roles both as a precursor to membrane lipids and as a signaling molecule. As more information on the roles of PAP in plants becomes available and the importance of PAP is revealed, protocols for assaying plant PAP...
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