Article
Flip-flop of fluorescently labeled phospholipids in proteoliposomes reconstituted with Saccharomyces cerevisiae microsomal proteins.
Eukaryotic cell - 1 Sept 2007
Vehring Stefanie, Pakkiri Leroy, Schröer Adrien, Alder-Baerens Nele, Herrmann Andreas, Menon Anant K, Pomorski Thomas
Abstract excerpt
A phospholipid flippase activity from the endoplasmic reticulum (ER) of the model organism Saccharomyces cerevisiae has been characterized and functionally reconstituted into proteoliposomes. Analysis of the transbilayer movement of acyl-7-nitrobenz-2-oxa-1,3-diazol-4-yl (acyl-NBD)-labeled phosph...
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