Article
Positive Feedback Genetic Circuit Incorporating a Constitutively Active Mutant Gal3 into Yeast GAL Induction System.
ACS synthetic biology - 16 Jun 2017
Ryo Shintaro, Ishii Jun, Matsuno Toshihide, Nakamura Yasuyuki, Matsubara Daiki, Tominaga Masahiro, Kondo Akihiko
Abstract excerpt
The GAL expression system is the most frequently used induction technique in the yeast Saccharomyces cerevisiae. Here we report a simple but powerful genetic circuit for use with the GAL induction system. Briefly, an artificial positive feedback circuit was incorporated into the GAL regulatory network. We selected green fluorescent protein (GFP) as a reporter of GAL1 induction, and designed a strain that...
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