Article
Systematic morphological profiling of human gene and allele function via Cell Painting.
eLife - 18 Mar 2017
Rohban Mohammad Hossein, Singh Shantanu, Wu Xiaoyun, Berthet Julia B, Bray Mark-Anthony, Shrestha Yashaswi, Varelas Xaralabos, Boehm Jesse S, Carpenter Anne E
Abstract excerpt
We hypothesized that human genes and disease-associated alleles might be systematically functionally annotated using morphological profiling of cDNA constructs, via a microscopy-based Cell Painting assay. Indeed, 50% of the 220 tested genes yielded detectable morphological profiles, which grouped into biologically meaningful gene clusters consistent with known functional annotation (e.g., the RAS-RAF-MEK-ERK...
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