Article
Phenotypic and functional consequences of different isolation protocols on skin mononuclear phagocytes.
Journal of leukocyte biology - 1 Jun 2017
Botting Rachel A, Bertram Kirstie M, Baharlou Heeva, Sandgren Kerrie J, Fletcher James, Rhodes Jake W, Rana Hafsa, Plasto Toby M, Wang Xin Maggie, Lim Jake J K, Barnouti Laith, Kohout Mark P, Papadopoulos Tim, Merten Steve, Olbourne Norman, Cunningham Anthony L, Haniffa Muzlifah, Harman Andrew N
Abstract excerpt
Mononuclear phagocytes are present in skin and mucosa and represent one of the first lines of defense against invading pathogens, which they detect via an array of pathogen-binding receptors expressed on their surface. However, their extraction from tissue is difficult, and the isolation technique used has functional consequences on the cells obtained. Here, we compare mononuclear phagocytes isolated from human...
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