Article
Isolation and Characterization of Mononuclear Phagocytes in the Mouse Lung and Lymph Nodes.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018
Gibbings Sophie L, Jakubzick Claudia V
Abstract excerpt
There is a diverse population of mononuclear phagocytes (MPs) in the lungs, comprised of macrophages, dendritic cells (DCs), and monocytes. The existence of these various cell types suggests that there is a clear division of labor and delicate balance between the MPs under steady-state and inflammatory conditions. Here we describe how to identify pulmonary MPs using flow cytometry and how to isolate them via cell...
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