Article
Enhanced CRISPR/Cas9-mediated biallelic genome targeting with dual surrogate reporter-integrated donors.
FEBS letters - 1 Mar 2017
Wu Yun, Xu Kun, Ren Chonghua, Li Xinyi, Lv Huijiao, Han Furong, Wei Zehui, Wang Xin, Zhang Zhiying
Abstract excerpt
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system has recently emerged as a simple, yet powerful genome engineering tool, which has been widely used for genome modification in various organisms and cell types. However, screening biallelic genome-modified cells is often time-consuming and technically challenging. In this study, we incorporated two...
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