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Article

Engineering of CYP106A2 for steroid 9α- and 6β-hydroxylation.

Steroids - 1 Apr 2017

Nikolaus Julia, Nguyen Kim Thoa, Virus Cornelia, Riehm Jan L, Hutter Michael, Bernhardt Rita

Abstract excerpt

CYP 106A2 from Bacillus megaterium ATCC 13368 has been described as a 15β-hydroxylase showing also minor 11α-, 9α- and 6β-hydroxylase activity for progesterone conversion. Previously, mutant proteins with a changed selectivity towards 11α-OH-progesterone have already been produced. The challenge of this work was to create mutant proteins with a higher regioselectivity towards hydroxylation at positions 9 and 6 of...

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Source

Identifiers

PMID: 28163026
DOI: 10.1016/j.steroids.2017.01.005

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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