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Article

Two strategies to engineer flexible loops for improved enzyme thermostability.

Scientific reports - 1 Feb 2017

Yu Haoran, Yan Yihan, Zhang Cheng, Dalby Paul A

Abstract excerpt

Flexible sites are potential targets for engineering the stability of enzymes. Nevertheless, the success rate of the rigidifying flexible sites (RFS) strategy is still low due to a limited understanding of how to determine the best mutation candidates. In this study, two parallel strategies were applied to identify mutation candidates within the flexible loops of Escherichia coli transketolase (TK). The first was...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 28145457
DOI: 10.1038/srep41212
PMCID: PMC5286519

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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