Article
Gradually accumulating beneficial mutations to improve the thermostability of N-carbamoyl-D-amino acid amidohydrolase by step-wise evolution.
Applied microbiology and biotechnology - 1 May 2011
Zhang Dalong, Zhu Fuyun, Fan Wenchao, Tao Rongsheng, Yu Hong, Yang Yunliu, Jiang Weihong, Yang Sheng
Abstract excerpt
To further enhance repeated batch reactions with immobilized N-carbamoyl-D-amino acid amidohydrolase (DCase), which can be used for the industrial production of D-amino acids, the stability of high soluble mutant DCase-M3 from Ralstonia pickettii CGMCC1596 was improved by step-wise evolution. In our previous report, six thermostability-related sites were identified by error-prone PCR. Based on the above result,...
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