Article
Sequencing protocols to genotype mdx, mdx(4cv), and mdx(5cv) mice.
Muscle & nerve - 1 Aug 2010
Banks Glen B, Combs Ariana C, Chamberlain Jeffrey S
Abstract excerpt
Currently available polymerase chain reaction (PCR) genotyping methods for point mutations in the mouse dystrophin gene can lead to false positives and result in wasted time and money due to breeding or treating the wrong mice. Here we describe a simple and accurate method for sequencing the point mutations in mdx, mdx(4cv), and mdx(5cv) mice. This method clearly distinguishes between wildtype, heterozygous, and...
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