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Article

Mutants of Ca2+ -regulated Photoprotein Obelin for Site-specific Conjugation.

Photochemistry and photobiology - 1 Mar 2017

Krasitskaya Vasilisa V, Burakova Ludmila P, Komarova Anastasia A, Bashmakova Eugenia E, Frank Ludmila A

Abstract excerpt

Color variants of Ca2+ -regulated photoprotein obelin were shown to be an important tool for dual-analyte binding assay. To provide site-directed conjugation with biospecific molecules, several obelin color mutants carrying unique cysteine residues were obtained and characterized for their novel properties. A pair of obelins Y138F,A5C and W92F,H22E,D12C was found to be most suitable (in terms of high...

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Identifiers

PMID: 28063150
DOI: 10.1111/php.12712

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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