Article
MSRE-HTPrimer: a high-throughput and genome-wide primer design pipeline optimized for epigenetic research.
Clinical epigenetics - 1 Jan 2016
Pandey Ram Vinay, Pulverer Walter, Walter Pulverer, Kallmeyer Rainer, Beikircher Gabriel, Pabinger Stephan, Kriegner Albert, Weinhäusel Andreas
Abstract excerpt
BACKGROUND: Methylation-sensitive restriction enzymes-polymerase chain reaction (MSRE-PCR) has been used in epigenetic research to identify genome-wide and gene-specific DNA methylation. Currently, epigenome-wide discovery studies provide many candidate regions for which the MSREqPCR approach can be very effective to confirm the findings. MSREqPCR provides high multiplexing capabilities also when starting with...
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