Article
Exploring STR signal in the single- and multicopy number regimes: Deductions from an in silico model of the entire DNA laboratory process.
Electrophoresis - 1 Mar 2017
Duffy Ken R, Gurram Neil, Peters Kelsey C, Wellner Genevieve, Grgicak Catherine M
Abstract excerpt
Short tandem repeat (STR) profiling from DNA samples has long been the bedrock of human identification. The laboratory process is composed of multiple procedures that include quantification, sample dilution, PCR, electrophoresis, and fragment analysis. The end product is a short tandem repeat electropherogram comprised of signal from allele, artifacts, and instrument noise. In order to optimize or alter...
Topics
- Alleles
- Computer Simulation
- DNA
- DNA Copy Number Variations
- DNA Fingerprinting
- Electrophoresis, Capillary
- Humans
- Microsatellite Repeats
- Polymerase Chain Reaction
- Sensitivity and Specificity