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Article

Universal Human Papillomavirus Typing Assay: Whole-Genome Sequencing following Target Enrichment.

Journal of clinical microbiology - 1 Mar 2017

Li Tengguo, Unger Elizabeth R, Batra Dhwani, Sheth Mili, Steinau Martin, Jasinski Jean, Jones Jennifer, Rajeevan Mangalathu S

Abstract excerpt

We designed a universal human papillomavirus (HPV) typing assay based on target enrichment and whole-genome sequencing (eWGS). The RNA bait included 23,941 probes targeting 191 HPV types and 12 probes targeting beta-globin as a control. We used the Agilent SureSelect XT2 protocol for library preparation, Illumina HiSeq 2500 for sequencing, and CLC Genomics Workbench for sequence analysis. Mapping stringency for...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 27974548
DOI: 10.1128/JCM.02132-16
PMCID: PMC5328449

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CLC

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