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Development and Validation of a New HPV Genotyping Assay Based on Next-Generation Sequencing

16 May 2014

Abstract excerpt

OBJECTIVES: We developed a new human papillomavirus (HPV) genotyping assay based on multiplex polymerase chain reaction and next-generation sequencing (NGS) methods for large-scale cervical cancer screening. METHODS: We first trained the assay on 1,170 self-collected samples, balancing the cutoff points for high-risk types. Then using 4,262 separate self-collected specimens, we compared concordance, sensitivity,...

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PMID: 24838323
DOI: 10.1309/ajcp9p2kjsxekcjb

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