Article
Repurposing the Hybrid Capture 2 (HC2) screening test for whole-genome sequencing of human papillomaviruses.
Archives of virology - 1 Dec 2021
Debernardi A, Jarassier W, Soret C, Mougin C, Alizon S, Bravo I G, Guenat D, Prétet J L, Lepiller Q
Abstract excerpt
Simple and standardized approaches for genome analysis of human papillomavirus (HPV) by next-generation sequencing are needed. The aim of the study was to develop a protocol for direct deep sequencing of high-risk (hr) HPV strains, based on the widely used commercial Hybrid Capture 2 (QIAGEN) test, without any additional probe design. This protocol was applied to 15 HPV-positive and two HPV-negative cervical...
Topics
- Alphapapillomavirus
- Cervix Uteri
- DNA, Viral
- Female
- Genotype
- Humans
- Papillomaviridae
- Papillomavirus Infections
- Sensitivity and Specificity
- Uterine Cervical Neoplasms