Article
Accurate human papillomavirus genotyping by 454 pyrosequencing.
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases - 1 Oct 2013
Militello V, Lavezzo E, Costanzi G, Franchin E, Di Camillo B, Toppo S, Palù G, Barzon L
Abstract excerpt
Accurate HPV typing is essential for evaluation and monitoring of HPV vaccines, for second-line testing in cervical cancer screening, and in epidemiological surveys. In this study, we set up and assessed in clinical samples a new HPV typing method based on 454 next-generation sequencing (NGS) of HPV L1 amplicons, generated by using a modified PGMY primer set with improved sensitivity for some HPV types that are...
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