Article
Evaluation of stability and validation of reference genes for RT-qPCR expression studies in rice plants under water deficit.
Journal of applied genetics - 1 May 2017
Auler Priscila Ariane, Benitez Letícia Carvalho, do Amaral Marcelo Nogueira, Vighi Isabel Lopes, Dos Santos Rodrigues Gabriela, da Maia Luciano Carlos, Braga Eugenia Jacira Bolacel
Abstract excerpt
Many studies use strategies that allow for the identification of a large number of genes expressed in response to different stress conditions to which the plant is subjected throughout its cycle. In order to obtain accurate and reliable results in gene expression studies, it is necessary to use reference genes, which must have uniform expression in the majority of cells in the organism studied. RNA isolation of...
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