Article
Normalization of reverse transcription quantitative-PCR with housekeeping genes in rice.
Biotechnology letters - 1 Nov 2003
Kim Bo-Ra, Nam Hee-Young, Kim Soo-Un, Kim Su-Il, Chang Yung-Jin
Abstract excerpt
Reverse transcription followed by real-time quantitative polymerase chain reaction (RT Q-PCR) is useful for the systematic measurement of plant physiological changes in gene expression. The validity of using 18S rRNA and three housekeeping genes, glyceraldehyde-3-phosphate dehydrogenase, actin, and tubulin, was tested as a reference of RT Q-PCR. Under various growth stages of etiolated seedlings, different...
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