Article
Selection of candidate reference genes for real-time PCR studies in lettuce under abiotic stresses.
Planta - 1 Jun 2014
Borowski Joyce Moura, Galli Vanessa, Messias Rafael da Silva, Perin Ellen Cristina, Buss Julieti Hugh, dos Anjos e Silva Sérgio Delmar, Rombaldi Cesar Valmor
Abstract excerpt
The process of selection and validation of reference genes is the first step in studies of gene expression by real-time quantitative polymerase chain reaction (RT-qPCR). The genome of lettuce, the most popular leaf vegetable cultivated worldwide, has recently been sequenced; therefore, suitable reference genes for reliable results in RT-qPCR analyses are required. In the present study, 17 candidate reference...
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