Article
Identification and Validation of Reference Genes and Their Impact on Normalized Gene Expression Studies across Cultivated and Wild Cicer Species.
PloS one - 1 Jan 2016
Reddy Dumbala Srinivas, Bhatnagar-Mathur Pooja, Reddy Palakolanu Sudhakar, Sri Cindhuri Katamreddy, Sivaji Ganesh Adusumalli, Sharma Kiran Kumar
Abstract excerpt
Quantitative Real-Time PCR (qPCR) is a preferred and reliable method for accurate quantification of gene expression to understand precise gene functions. A total of 25 candidate reference genes including traditional and new generation reference genes were selected and evaluated in a diverse set of chickpea samples. The samples used in this study included nine chickpea genotypes (Cicer spp.) comprising of...
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