Article
Reference genes for accurate transcript normalization in citrus genotypes under different experimental conditions.
PloS one - 1 Jan 2012
Mafra Valéria, Kubo Karen S, Alves-Ferreira Marcio, Ribeiro-Alves Marcelo, Stuart Rodrigo M, Boava Leonardo P, Rodrigues Carolina M, Machado Marcos A
Abstract excerpt
Real-time reverse transcription PCR (RT-qPCR) has emerged as an accurate and widely used technique for expression profiling of selected genes. However, obtaining reliable measurements depends on the selection of appropriate reference genes for gene expression normalization. The aim of this work w...
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