Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Copy number analysis by low coverage whole genome sequencing using ultra low-input DNA from formalin-fixed paraffin embedded tumor tissue.

Genome medicine - 15 Nov 2016

Kader Tanjina, Goode David L, Wong Stephen Q, Connaughton Jacquie, Rowley Simone M, Devereux Lisa, Byrne David, Fox Stephen B, Mir Arnau Gisela, Tothill Richard W, Campbell Ian G, Gorringe Kylie L

Abstract excerpt

Unlocking clinically translatable genomic information, including copy number alterations (CNA), from formalin-fixed paraffin-embedded (FFPE) tissue is challenging due to low yields and degraded DNA. We describe a robust, cost-effective low-coverage whole genome sequencing (LC WGS) method for CNA detection using 5 ng of FFPE-derived DNA. CN profiles using 100 ng or 5 ng input DNA were highly concordant and...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Carcinoma, Merkel Cell
Share to Cost-Benefit Analysis

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA Copy Number Variations

Share to DNA Repair

Share to Formaldehyde

Share to Gene Dosage

Share to Gene Library

Share to Oligonucleotide Array Sequence Analysis

Source

Open in PubMed Open full text

Identifiers

PMID: 27846907
DOI: 10.1186/s13073-016-0375-z
PMCID: PMC5111221

Genes

MIP

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Copy number analysis by low coverage whole genome sequencing using ultra low-input DNA from formalin-fixed paraffin embedded tumor tissue.PMID 27846907Read ↗

Shallow whole genome sequencing for robust copy number profiling of formalin-fixed paraffin-embedded breast cancersSemantic proximity Read ↗

Whole-genome single-cell copy number profiling from formalin-fixed paraffin-embedded samplesSemantic proximity Read ↗

Comprehensive Screening of Gene Copy Number Aberrations in Formalin-Fixed, Paraffin-Embedded Solid Tumors Using Molecular Inversion Probe-Based Single-Nucleotide Polymorphism Array.Semantic proximity Read ↗

Functional DNA quantification guides accurate next-generation sequencing mutation detection in formalin-fixed, paraffin-embedded tumor biopsiesSemantic proximity Read ↗

CNA Explorer and anaLyzer (CNAEL): an interactive web application and standard operating procedure enabling efficient clinical review and reporting of complex NGS-derived tumor copy number profilesSemantic proximity Read ↗

Crucial parameters for precise copy number variation detection in formalin-fixed paraffin-embedded solid cancer samples.Semantic proximity Read ↗

Clinical whole-genome sequencing from routine formalin-fixed, paraffin-embedded specimens: pilot study for the 100,000 Genomes Project.Semantic proximity Read ↗

A computational tool to detect DNA alterations tailored to formalin-fixed paraffin-embedded samples in cancer clinical sequencingSemantic proximity Read ↗

Detection of genome-wide copy number variants in myeloid malignancies using next-generation sequencing.Semantic proximity Read ↗

Integrated genomic DNA/RNA profiling of hematologic malignancies in the clinical setting.Semantic proximity Read ↗

Sensitive detection of copy number alterations in samples with low circulating tumor DNA fractionSemantic proximity Read ↗

Systematic identification of mutations and copy number alterations associated with cancer patient prognosis.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.