Article
Improved High-Throughput Sequencing of the Human Oral Microbiome: From Illumina to PacBio
11 Dec 2020
Abstract excerpt
BACKGROUND: . Five preschool children with no symptoms of oral and systemic diseases were recruited. Samples of saliva were collected. A 468 bp insert size library was constructed on the MiSeq platform and then subjected to 300 bp paired-end sequencing. Libraries with longer insert sizes, including a full-length 16S rDNA gene, were sequenced on the PacBio RS II platform. RESULTS: A total of 122.6 Mb of raw data,...
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