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Article

A high throughput targeted gene disruption method for Alternaria brassicicola functional genomics using linear minimal element (LME) constructs.

Molecular plant-microbe interactions : MPMI - 1 Jan 2006

Cho Yangrae, Davis Joshua W, Kim Kwang-Hyung, Wang Juan, Sun Qi-Hong, Cramer Robert A, Lawrence Christopher B

Abstract excerpt

Alternaria brassicicola causes black spot disease of cultivated Brassicas and has been used consistently as a necrotrophic fungal pathogen for studies with Arabidopsis. In A. brassicicola, mutant generation has been the most rate-limiting step for the functional analysis of individual genes due to low efficiency of both transformation and targeted integration. To improve the targeted gene disruption efficiency as...

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PMID: 16404948
DOI: 10.1094/MPMI-19-0007

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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