Article
Probing bulky ligand entry in engineered archaeal ferritins.
Biochimica et biophysica acta. General subjects - 1 Feb 2017
Calisti Lorenzo, Benni Irene, Cardoso Trabuco Matilde, Baiocco Paola, Ruzicka Barbara, Boffi Alberto, Falvo Elisabetta, Malatesta Francesco, Bonamore Alessandra
Abstract excerpt
BACKGROUND: A set of engineered ferritin mutants from Archaeoglobus fulgidus (Af-Ft) and Pyrococcus furiosus (Pf-Ft) bearing cysteine thiols in selected topological positions inside or outside the ferritin shell have been obtained. The two apo-proteins were taken as model systems for ferritin internal cavity accessibility in that Af-Ft is characterized by the presence of a 45Å wide aperture on the protein surface...
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